The careful design and proprietary software algorithms in the ChemiDoc MP System yield uniform data for every application, in every position. Putting It All Together The New ChemiDoc MP Imaging System This new coating also provides resistance to solarization, in which a material exhibits changes in color or emissivity after exposure to ultraviolet light, but can also progressively degrade the physical or mechanical properties of many materials, especially polymers, after prolonged exposure. This results in lower overall sensitivity of the system.įor the ChemiDoc MP System, we selected materials and coatings for the interior surfaces specifically to reduce fluorescence and thus eliminate this source of background. If any materials show autofluorescence and emit light within the bandpass of the emission filters, then background levels of the image will increase. With fluorescent western blots, the blot and some of the interior of the instrument is subjected to intense excitation light. Many materials will fluoresce when exposed to high intensity light, termed autofluorescence. Would have caused increased background in the images. Time caused the uncoated part of this component to fluoresce, which Our R&D team has done as much of that work for you as possible, by examining all possible sources of error then refining and optimizing the components, optics, electronics, and software to yield consistent results.Īutofluorescenece Suppression: Solarization due to UV exposure over For example, will you get the same results when a blot is placed on different areas of the platen? Imaging is an experiment too, and needs experimental controls in place for accuracy. The end user generally assumes that an instrument just works the way it’s supposed to.īut that can be a risky assumption, and doing good science means accounting for all sources of error. Researchers focused on their biological questions shouldn't also need to be experts in imaging. While obvious imaging artifacts are easily noticed, faint or missing bands, poor sensitivity, inconsistent results, inaccurate quantitation, and other issues may be mistaken as simply the way an instrument functions. Limitations in an imaging system may not be readily apparent to the user. See what differentiates a merely good imager from a truly great imaging system. Learn how Bio-Rad imaging systems are custom- designed for peak performance from the leaders of our Imaging R&D team.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |